Skip to main content

Links between DNA methylation and nucleosome occupancy in the human genome Clayton K. Collings and John N. Anderson


Epigenetics & Chromatin  2017  10:18  DOI: 10.1186/s13072-017-0125-5

 Previous studies from our laboratory have shown that DNA methylation directly enhances the stability of nucleosomes in vitro.  However, the relationship between DNA methylation and nucleosome occupancy in the cell is poorly understood. In this study, we implemented a bioinformatics approach to study links between DNA methylation and nucleosome occupancy throughout the entire human genome. Using this approach, we demonstrated that increasing  methylated CpG density is correlated with nucleosome occupancy and that in methylated CpG-rich nucleosomes, methylation levels are greater in the core than in the adjacent linker DNA. These nucleosomal DNA methylation patterns were detected not only in total genomic DNA but also within most subgenomic regions. Prominent exceptions to the positive correlation between mCpG density and nucleosome occupancy included CpG islands marked by H3K27me3 and CpG-poor heterochromatin marked by H3K9me3, and these modifications, along with DNA methylation, are the major silencing mechanisms of the human genome. 

Purdue University Biological Sciences, 915 Mitch Daniels Boulevard, West Lafayette, IN 47907

Main Office: (765) 494-4408   Business Office: (765) 494-4764  Contact Us

© 2024 Purdue University | An equal access/equal opportunity university | Copyright Complaints

Trouble with this page? Disability-related accessibility issue? Please contact the College of Science Webmaster.

Maintained by Science IT