GROWTH MEDIA FOR AGROBACTERIUM

YEP

Per liter:

Bacto peptone    10 g
NaCl                   5 g
Yeast extract      10 g
(Agar)               15 g
No pH adjustment

AB Buffer Per liter (20x stock solution):

K₂HPO₄                    60 g
(or K₂HPO₄.3H₂O      78.6 g)
NaH₂PO₄                   20 g
(or NaH₂PO₄.H₂0       23 g)

AB Salts Per liter (20x stock solution):

NH₄Cl                      20 g
MgSO₄.7H₂0              6 g
(or MgSO₄               2.9 g)
KCl                            3 g
CaCl₂                       0.2 g
(or CaCl₂.2H₂0        0.26 g)
FeSO₄.7H₂0              50 mg

Sterilize the AB Buffer and the AB Salts separately. Shake the salts before use to disperse the FeSO₄.

Add sucrose to a final concentration of 0.5%.

This work is made possible by a grant from the National Science Foundation's Plant Genome Research Program.

Questions or Comments regarding this web site should be addressed to Dr. Stanton Gelvin