Posts tagged ‘iPSCs’

Eye diseases

  1. Lin H, Ouyang H, Zhu J, Huang S, Liu Z, Chen S, Cao G, Li G, Signer RA, Xu Y, Chung C, Zhang Y, Lin D, Patel S, Wu F, Cai H, Hou J, Wen C, Jafari M, Liu X, Luo L, Zhu J, Qiu A, Hou R, Chen B, Chen J, Granet D, Heichel C, Shang F, Li X, Krawczyk M, Skowronska-Krawczyk D, Wang Y, Shi W, Chen D, Zhong Z, Zhong S, Zhang L, Chen S, Morrison SJ, Maas RL, Zhang K, Liu Y. Lens regeneration using endogenous stem cells with gain of visual function. Nature. 2016 Mar 9. doi: 10.1038/nature17181. [Epub ahead of print] PubMed PMID: 26958831. News release: Stem Cells Regenerate Human Lens After Cataract Surgery, Restoring Vision
  2. Hayashi R, Ishikawa Y, Sasamoto Y, Katori R, Nomura N, Ichikawa T, Araki S, Soma T, Kawasaki S, Sekiguchi K, Quantock AJ, Tsujikawa M, Nishida K. Co-ordinated ocular development from human iPS cells and recovery of corneal function. Nature. 2016 Mar 9. doi: 10.1038/nature17000. [Epub ahead of print] PubMed PMID: 26958835.

Stem cells

  1. Sridhar A, Ohlemacher SK, Langer KB, Meyer JS. Robust Differentiation of mRNA-Reprogrammed Human Induced Pluripotent Stem Cells Toward a Retinal Lineage. Stem Cells Transl Med. 2016 Mar 1. pii: sctm.2015-0093. [Epub ahead of print] PubMed PMID: 26933039.
  2. Sagi I, Chia G, Golan-Lev T, Peretz M, Weissbein U, Sui L, Sauer MV, Yanuka O, Egli D, Benvenisty N. Derivation and differentiation of haploid human embryonic stem cells. Nature. 2016 Mar 16. doi: 10.1038/nature17408. [Epub ahead of print] PubMed PMID: 26982723.

Development

  1. Pueschel R, Coraggio F, Meister P. From single genes to entire genomes: the search for a function of nuclear organization. Development. 2016 Mar 15;143(6):910-23. doi: 10.1242/dev.129007. Review. PubMed PMID: 26980791.
  2. Salomon DR. A CRISPR Way to Block PERVs – Engineering Organs for Transplantation. N Engl J Med. 2016 Mar 17;374(11):1089-1091. PubMed PMID: 26981939.

Neuroscience

  1. Mullins C, Fishell G, Tsien RW. Unifying Views of Autism Spectrum Disorders: A Consideration of Autoregulatory Feedback Loops. Neuron. 2016 Mar 16;89(6):1131-56. doi: 10.1016/j.neuron.2016.02.017. Review. PubMed PMID: 26985722.
  2. Hazen JL, Faust GG, Rodriguez AR, Ferguson WC, Shumilina S, Clark RA, Boland MJ, Martin G, Chubukov P, Tsunemoto RK, Torkamani A, Kupriyanov S, Hall IM, Baldwin KK. The Complete Genome Sequences, Unique Mutational Spectra, and Developmental Potency of Adult Neurons Revealed by Cloning. Neuron. 2016 Mar 16;89(6):1223-36. doi: 10.1016/j.neuron.2016.02.004. Epub 2016 Mar 3. PubMed PMID: 26948891; PubMed Central PMCID: PMC4795965.

Mathematics

  1. Castelvecchi D. Fermat’s last theorem earns Andrew Wiles the Abel Prize. Nature. 2016 Mar 17;531(7594):287. doi: 10.1038/nature.2016.19552. PubMed PMID: 26983518.

Genomics

  1. Bintu L, Yong J, Antebi YE, McCue K, Kazuki Y, Uno N, Oshimura M, Elowitz MB. Dynamics of epigenetic regulation at the single-cell level. Science. 2016 Feb 12;351(6274):720-4. doi: 10.1126/science.aab2956. PubMed PMID: 26912859.
    • Commentary: Keung AJ, Khalil AS. MOLECULAR BIOLOGY. A unifying model of epigenetic regulation. Science. 2016 Feb 12;351(6274):661-2. doi: 10.1126/science.aaf1647. PubMed PMID: 26912843.
  2. Cahan P. Enabling direct fate conversion with network biology. Nat Genet. 2016 Feb 24;48(3):226-7. doi: 10.1038/ng.3516. PubMed PMID: 26906680.
  3. Rackham OJ, Firas J, Fang H, Oates ME, Holmes ML, Knaupp AS; FANTOM Consortium, Suzuki H, Nefzger CM, Daub CO, Shin JW, Petretto E, Forrest AR, Hayashizaki Y, Polo JM, Gough J. A predictive computational framework for direct reprogramming between human cell types. Nat Genet. 2016 Jan 18. doi: 10.1038/ng.3487. [Epub ahead of print] PubMed PMID: 26780608.
  4. Savova V, Chun S, Sohail M, McCole RB, Witwicki R, Gai L, Lenz TL, Wu CT, Sunyaev SR, Gimelbrant AA. Genes with monoallelic expression contribute disproportionately to genetic diversity in humans. Nat Genet. 2016 Jan 25. doi: 10.1038/ng.3493. [Epub ahead of print] PubMed PMID: 26808112.
  5. Gusev A, Ko A, Shi H, Bhatia G, Chung W, Penninx BW, Jansen R, de Geus EJ, Boomsma DI, Wright FA, Sullivan PF, Nikkola E, Alvarez M, Civelek M, Lusis AJ, Lehtimäki T, Raitoharju E, Kähönen M, Seppälä I, Raitakari OT, Kuusisto J, Laakso M, Price AL, Pajukanta P, Pasaniuc B. Integrative approaches for large-scale transcriptome-wide association studies. Nat Genet. 2016 Feb 8. doi: 10.1038/ng.3506. [Epub ahead of print] PubMed PMID: 26854917.

Development

  1. Aggarwal V, Dickinson RB, Lele TP. Concentration Sensing by the Moving Nucleus in Cell Fate Determination: A Computational Analysis. PLoS One. 2016 Feb 12;11(2):e0149213. doi: 10.1371/journal.pone.0149213. eCollection 2016. PubMed PMID: 26872214.
  2. Rackham OJ, Firas J, Fang H, Oates ME, Holmes ML, Knaupp AS; FANTOM Consortium, Suzuki H, Nefzger CM, Daub CO, Shin JW, Petretto E, Forrest AR, Hayashizaki Y, Polo JM, Gough J. A predictive computational framework for direct reprogramming between human cell types. Nat Genet. 2016 Jan 18. doi: 10.1038/ng.3487. [Epub ahead of print] PubMed PMID: 26780608.

Molecular Biology

  1. Burgers PM, Gordenin D, Kunkel TA. Who Is Leading the Replication Fork, Pol ε or Pol δ? Mol Cell. 2016 Feb 18;61(4):492-3. doi: 10.1016/j.molcel.2016.01.017. PubMed PMID: 26895421.
  2. Johnson RE, Klassen R, Prakash L, Prakash S. Response to Burgers et al. Mol Cell. 2016 Feb 18;61(4):494-5. doi: 10.1016/j.molcel.2016.01.018. PubMed PMID: 26895422.

Funding

  1.  Vachelard J, Gambarra-Soares T, Augustini G, Riul P, Maracaja-Coutinho V. A Guide to Scientific Crowdfunding. PLoS Biol. 2016 Feb 17;14(2):e1002373. doi: 10.1371/journal.pbio.1002373. eCollection 2016 Feb. PubMed PMID: 26886064.

Genome editing

  1. Tai DJ, Ragavendran A, Manavalan P, Stortchevoi A, Seabra CM, Erdin S, Collins RL, Blumenthal I, Chen X, Shen Y, Sahin M, Zhang C, Lee C, Gusella JF, Talkowski ME. Engineering microdeletions and microduplications by targeting segmental duplications with CRISPR. Nat Neurosci. 2016 Feb 1. doi: 10.1038/nn.4235. [Epub ahead of print] PubMed PMID: 26829649.

Behaviour

  1. Scott CA, Marsden AN, Slusarski DC. Automated, high-throughput, in vivo analysis of visual function using the zebrafish. Dev Dyn. 2016 Feb 18. doi: 10.1002/dvdy.24398. [Epub ahead of print] PubMed PMID: 26890697.

Stem cells

  1. Fatehullah A, Tan SH, Barker N. Organoids as an in vitro model of human development and disease. Nat Cell Biol. 2016 Feb 25;18(3):246-54. doi: 10.1038/ncb3312. Review. PubMed PMID: 26911908.
  2. Wang Y, Gao S. Human Naive Embryonic Stem Cells: How Full Is the Glass? Cell Stem Cell. 2016 Mar 3;18(3):301-3. doi: 10.1016/j.stem.2016.02.012. PubMed PMID: 26942847.
  3. Pastor WA, Chen D, Liu W, Kim R, Sahakyan A, Lukianchikov A, Plath K, Jacobsen SE, Clark AT. Naive Human Pluripotent Cells Feature a Methylation Landscape Devoid of Blastocyst or Germline Memory. Cell Stem Cell. 2016 Mar 3;18(3):323-9. doi: 10.1016/j.stem.2016.01.019. Epub 2016 Feb 4. PubMed PMID: 26853856; PubMed Central PMCID: PMC4779431.
  4. Passier R, Orlova V, Mummery C. Complex Tissue and Disease Modeling using hiPSCs. Cell Stem Cell. 2016 Mar 3;18(3):309-21. doi: 10.1016/j.stem.2016.02.011. Review. PubMed PMID: 26942851.
  5. Orlova V, Mummery C. SnapShot: Key Advances in hiPSC Disease Modeling. Cell Stem Cell. 2016 Mar 3;18(3):422. doi: 10.1016/j.stem.2016.02.013. PubMed PMID: 26942854.
  6. Nat Rev Mol Cell Biol; Focus on: Pluripotent Stem cells

Medicine

  1. Peplow M. Synthetic biology’s first malaria drug meets market resistance. Nature. 2016 Feb 25;530(7591):389-90. doi: 10.1038/530390a. PubMed PMID: 26911755.

Genome editing

  1. Hayes RP, Xiao Y, Ding F, van Erp PB, Rajashankar K, Bailey S, Wiedenheft B, Ke A. Structural basis for promiscuous PAM recognition in type I-E Cascade from E. coli. Nature. 2016 Feb 10. doi: 10.1038/nature16995. [Epub ahead of print] PubMed PMID: 26863189.

This is a collection of the videos that I used in lab discussion section of the ethical issues in human embryonic stem cells research in the 2014 BIOL36701 Principles Development at Purdue University.

1. An introduction to stem cell – A stem cell story

2. Are embryos persons?

3. A question of balance?

4. How patients may be exploited – Stem cell fraud

5. hESCs alternative I – Stem cells – the future: an introduction to iPS cells

6. hESCs alternative II – STAP cells

Stem cells

  1. Hou P, Li Y, Zhang X, Liu C, Guan J, Li H, Zhao T, Ye J, Yang W, Liu K, Ge J, Xu J, Zhang Q, Zhao Y, Deng H. Pluripotent stem cells induced from mouse somatic cells by small-molecule compounds. Science. 2013 Aug 9;341(6146):651-4. doi: 10.1126/science.1239278. Epub 2013 Jul 18. PubMed PMID: 23868920.

Neuroscience

  1. Shomrat T, Levin M. An automated training paradigm reveals long-term memory in planaria and its persistence through head regeneration. J Exp Biol. 2013 Jul 2. [Epub ahead of print] PubMed PMID: 23821717.
  2. Esposti F, Johnston J, Rosa JM, Leung KM, Lagnado L. Olfactory Stimulation Selectively Modulates the OFF Pathway in the Retina of Zebrafish. Neuron. 2013 Jul 10;79(1):97-110. doi: 10.1016/j.neuron.2013.05.001. PubMed PMID: 23849198.
    • Commentary: “Dowling JE. Olfaction and Vision Meet in the Retina. Neuron. 2013 Jul 10;79(1):1-3. doi: 10.1016/j.neuron.2013.06.024. PubMed PMID: 23849191.

Retina

  1. Endeman D, Klaassen LJ, Kamermans M. Action spectra of zebrafish cone photoreceptors. PLoS One. 2013 Jul 5;8(7):e68540. doi: 10.1371/journal.pone.0068540. Print 2013. PubMed PMID: 23861916; PubMed Central PMCID: PMC3702584.

Genetics

  1. Poznik GD, Henn BM, Yee MC, Sliwerska E, Euskirchen GM, Lin AA, Snyder M, Quintana-Murci L, Kidd JM, Underhill PA, Bustamante CD. Sequencing Y chromosomes resolves discrepancy in time to common ancestor of males versus females. Science. 2013 Aug 2;341(6145):562-5. doi: 10.1126/science.1237619. PubMed PMID: 23908239.
  2. Francalacci P, Morelli L, Angius A, Berutti R, Reinier F, Atzeni R, Pilu R, Busonero F, Maschio A, Zara I, Sanna D, Useli A, Urru MF, Marcelli M, Cusano R, Oppo M, Zoledziewska M, Pitzalis M, Deidda F, Porcu E, Poddie F, Kang HM, Lyons R, Tarrier B, Gresham JB, Li B, Tofanelli S, Alonso S, Dei M, Lai S, Mulas A, Whalen MB, Uzzau S, Jones C, Schlessinger D, Abecasis GR, Sanna S, Sidore C, Cucca F. Low-pass DNA sequencing of 1200 Sardinians reconstructs European Y-chromosome phylogeny. Science. 2013 Aug 2;341(6145):565-9. doi: 10.1126/science.1237947. PubMed PMID: 23908240.
  3. Fu Y, Foden JA, Khayter C, Maeder ML, Reyon D, Joung JK, Sander JD. High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells. Nat Biotechnol. 2013 Jun 23. doi: 10.1038/nbt.2623. [Epub ahead of print] PubMed PMID: 23792628.

was awarded jointly to Sir John Gurdon and Shinya Yamnaka

for the discovery that mature cells can be reprogrammed to become pluripotent

They also won the Lasker Award in 2009.

 

Over the years, I have been leading a discussion about the ethical and social issues of stem cells research in my class and have used a few useful videos on various aspects. You may find them useful too.

I. Introduction to the topic

References

Here are two recent scientific references that give the current status of iPSCs research and compared that with hESCs, as well as the challenges ahead in research and ethical issues.

  1. Robinton DA, Daley GQ. The promise of induced pluripotent stem cells in research and therapy. Nature. 2012 Jan 18;481(7381):295-305. PubMed PMID: 22258608.
  2. Hug K, Hermerén G. Do we still need human embryonic stem cells for stem cell-based therapies? Epistemic and ethical aspects. Stem Cell Rev. 2011 Nov;7(4):761-74. PubMed PMID: 21461713.

This reference is a general scientific review on various ways that one can reprogramming cells that may potentially be used for therapeutic purposes.

  1.  Gurdon JB, Melton DA. Nuclear reprogramming in cells. Science. 2008 Dec 19;322(5909):1811-5. Review. PubMed PMID: 19095934.