Archive for the ‘lab activities’ Category

Prahatha Venkatraman and Yuk Fai Leung attended the 2015 Annual Meeting of the Association for Research in Vision and Ophthalmology (ARVO) in Denver, CO, from 5/3 5/7.

ARVO 2015

ARVO 2015

Prahatha presented a poster entitled “Understanding the contribution of photoreceptors to the visual motor response”.

Prahatha and Fai

Prahatha presenting her poster to colleagues, including Dr. Stephan Neuhauss on the left.

Lue Xiang, a former visiting scholar in the laboratory, also attended the meeting and presented a poster entitled “Charaterization of a retinitis pigmentosa gene slc7a14 in zebrafish by expression analysis and targeted deletion with CRISPR-Cas”.

Lue

Lilly Hall of Science; 2015 Purdue Spring Fest

Prahatha Venkatraman showing the way to measure fish vision to a future scientist.

Martian Tian explaining how to use zebrafish for eye disease research to the Lafayette community.

Saturday, June 14th marked the end of my first week at Woods Hole (I apologize for the late post). It was certainly a genomics bootcamp! In just one week, we learnt to dissect the ventral nerve chords from 3rd instar Drosophila larvae, extracted RNA, did microarrays, analyzed data and came up with hypotheses of what could be happening in our samples. We interacted and worked closely with wonderful TAs and faculty from different universities. This exercise certainly made me appreciate the hard work put in to genomic techniques (especially microarrays, RNAseq) and the enormity of the data obtained.

To celebrate the end of first week, some of us took a ferry to Martha’s Vineyard before charging up for the next section.

On the Ferry to Martha's Vineyard

We rented bikes and explored the beautiful island a little bit before settling for a nice picnic on the beach!

America's oldest platform carousel at Martha's Vineyard

Exploring Martha's Vineyard

Thus ended a great first week.
Next post: EPhys !

Lipid synthesis, stem cells, CRISPR, cell differentiation, coffee, faculty life in Puerto Rico, softball, ion channels, PhD in Europe, ball room dancing, buying a house in seattle, Epilepsy, larval Drosophila brain dissection – that’s an eclectic mix of topics to talk about for one day and this is not even an exhaustive list! These topics pretty much sum up my first day here at Woods Hole, where I am a part of the Neurobiology summer course at MBL with 13 other students from different parts of the US and Europe. I am housed at the Ebert hall with another student from the same course. The view from our room is gorgeous!

View from my room overlooking the Eel Pond

We started the day with an orientation/welcome brunch at the swope center. I was pleasantly surprised with the variety of food and taste. For cafeteria food, it was really good. There was everything from cereal, to yogurt parfait, to your-style-eggs and toast, waffles, fruits and vegetables, juice and coffee. There was even ice cream at 9 am! Despite the scorching heat, after the orientation my roommate and I went exploring this quaint nautical town with several beautiful small beaches.

DNA Man

I also just had to try the coffee at the local cafe.

Ok, not an image of coffee. But this was an unusual yet appropriate sign at the cafe

We had to get back to our ‘base’ because of the heat but we got some rest before the course’s first meeting in the afternoon. This session was interesting and we jumped right into a lecture about ion channels and Graeme Davis, one of the directors of the course, outlined some of the questions we are going to work on in the following week. After the lecture, we headed to the lab where I met the larval drosophila for the first time. I have dissected testes from adult flies before, but today we learnt to dissect brain from 3rd instar larvae. The larvae were expressing GFP in their motorneurons which let us take a quick look under fluorescence to see if we destroyed the brain completely or managed to successfully practice isolating the tissue for FACS sorting tomorrow.

GFP expressing motor neurons in Drosophila larvae. I did not do this btw.

The day ended with passionate discussions about science interspersed with pizza and beer!

A fun comic about everyday happenings in the Leung lab.












The members of the Leung Lab gathered to celebrate the Christmas and New Year holidays on 12/6/2013. Here are some pictures (Courtesy of Lue Xiang).

you may get a pleasant surprise when you come back!

It looks like that a Christmas/New Year ritual has been established in the lab….

Sylvia and Fai attended the 2013 Annual Meeting of the Association for Research in Vision and Ophthalmology and presented their work on novel signal transduction for photoreceptor development and using traditional Chinese medicine to treat retinal degeneration. Sylvia received a National Eye Institute Travel Grant and Yeunkyung Woo Achieve Excellence Travel Award to attend the meeting. Sylvia and Fai also interacted with colleagues all over the world during different events including the SERI reception, ARVO dinner with The Eye Hospital of Wenzhou Medical College, and ARVO CUHK-JSIEC Research Networking Evening.

Sylvia and Fai in front of Sylvia’s poster

Picture opportunity with the hosts of ARVO CUHK-JSIEC Research Networking Evening
From Left to Right: Yuk Fai Leung (Purdue), Prof. Mingzhi Zhang (Joint Shantou International Eye Center), and Prof. Calvin Pang (Chinese University of Hong Kong)

We have got an opportunity to share the vision on using zebrafish for screening eye drugs in our JSIEC-Purdue Joint Laboratory with many distinguished guests from all over the world, including Dr. Mansoor Sarfarazi (U Conn), Dr. G Kumaramanickavel (Narayana Nethralaya), Dr. John Prakash (Associate Director, NEI), Dr. Martine Jager (Former ARVO president, Leiden University), Dr. Motokazu Tsujikawa (Osaka), Dr.Vincent Raymond (U Laval) , Dr. Kanxing Zhao (Zhongshan) and many more.

Fai and Sylvia with Dr. Mingzhi Zhang (JSIEC), Dr. Haoyu Chen (JSIEC), Dr. John Prakash (NEI) and Mrs. Prakash.

you may get a pleasant surprise when you come back!