John Tesmer Publications

Journal

Waldschmidt, H. V., Homan, K. T., Cato, M. C., Cruz-Rodríguez, O., Cannavo, A., Wilson, M. W., Song, J., Cheung, J. Y., Koch, W. J., Tesmer, J. J. G., Larsen, S. D. 2017. Structure-based design of highly selective and potent G protein-coupled receptor kinase 2 inhibitors based on paroxetine. J Med Chem. (Epub ahead of print.)                doi: 10.1021/acs.jmedchem.7b00112. 

Chen, Q., Tesmer, J. J. G. 2017. A receptor on acid. Cell 168:339-341.

Tesmer, J. J. G. 2016. Hitchhiking on the heptahelical highway: structure and function of 7TM receptor complexes. Nat Rev Mol Cell Biol. 17:439-450.

Yang, P., Homan, K. T., Li, Y., Cruz-Rodríguez, O., Tesmer, J. J. G., Chen, Z. 2016. Effect of lipid composition on membrane orientation of the G protein-coupled receptor kinase 2—Gβ1γ2 complex. Biochemistry 55:2841-2848.

Cash, J. N., Davis, E. M., Tesmer, J. J. G. 2016. Structural and biochemical characterization of the catalytic core of the metastatic factor P-Rex1 and its regulation by PtdIns(3,4,5)P3. Structure 24:730-740.

Wang, Y., Virtanen, J., Xue, Z., Tesmer, J. J. G., Zhang, Y. 2016. Using iterative fragment assembly and progressive sequence truncation to facilitate phasing and crystal structure determination of distantly related proteins. Acta Cryst D Struct Biol. 72:616-628.

Waldschmidt, H. V., Homan, K. T., Cruz-Rodríguez, O., Cato, M. C., Waninger-Saroni, J., Larimore, K. M., Cannavo, A., Song, J., Cheung, J. Y., Kirchhoff, P. D., Koch, W. J., Tesmer, J. J. G., Larsen, S. D. 2016. Structure-based design, synthesis and biological evaluation of highly selective and potent G protein-coupled receptor kinase 2 inhibitors. J Med Chem. 59:3793-3807.

Taylor, V. G., Bommarito, P. A., Tesmer, J. J. G. 2016. Structure of the regulator of G protein signaling 8 (RGS8)–Gαq complex: molecular basis for Gα selectivity. J Biol Chem. 291:5138-5145.

Abe, A., Hiraoka, M., Ohguro, H., Tesmer, J. J. G., Shayman, J. A. 2016. Preferential hydrolysis of truncated oxidized glycerophospholipids by lysosomal phospholipase A2. J Lipid Res. 58:339-349.

Homan, K. T., Waldschmidt, H. V., Glukhova, A., Cannavo, A., Song, J., Cheung, J. Y., Koch, W. J., Larsen, S. D., Tesmer, J. J. G. 2015. Crystal structure of G protein-coupled receptor kinase 5 in complex with a rationally designed inhibitor J Biol Chem. 290:20649-20659. (Selected as “Paper of the Week”)

Inagaki, S., Ghirlando, R., Vishnivetskiy, S. A., Homan, K. T., White, J. F., Tesmer, J. J. G., Gurevich, V. V., Grisshammer, R. 2015. G protein-coupled receptor kinase 2 (GRK2) and 5 (GRK5) exhibit selective phosphorylation of the neurotensin receptor in vitro. Biochemistry 54:4320-4329.

Li, L., Homan, K. T., Vishnivetskiy, S. A., Manglik, A., Tesmer, J. J. G., Gurevich, V. V., Gurevich, E. V. 2015. G protein-coupled receptor kinases of the GRK4 subfamily phosphorylate inactive G protein-coupled receptors (GPCRs). J Biol Chem. 290:10775-10790.

Azevedo, A. W., Doan, T., Moaven, H., Sokal, I., Baameur, F., Vishnivetskiy, S. A., Homan, K. T., Tesmer, J. J. G., Gurevich, V. V., Chen, J., Rieke, F. 2015. C-terminal threonines and serines play distinct roles in the desensitization of rhodopsin, a G protein-coupled receptor. Elife 4:e05981.

Schumacher, S. M., Gao, E., Zhu, W., Chen, X., Chuprun, J. K., Feldman, A. M., Tesmer, J. J. G., Koch, W. J. 2015. Paroxetine-mediated GRK2 inhibition reverses cardiac dysfunction and remodeling after myocardial infarction. Sci Transl Med. 7:277ra31.

Glukhova, A., Hinkovska-Galcheva, V., Kelly, R., Abe, A., Shayman, J. A., Tesmer, J. J. G. 2015. Structure and function of lysosomal phospholipase A2 and lecithin:cholesterol acyltransferase. Nat Commun. 6:6250.

Homan, K. T., Larimore, K. M., Elkins, J. M., Szklarz, M., Knapp, S., Tesmer, J. J. G. 2015. Identification and structure-function analysis of subfamily selective G protein-coupled receptor kinase inhibitors. ACS Chem Biol. 10:310-319.

Homan, K. T., Tesmer, J. J. G. 2015. Molecular basis for small molecule inhibition of G protein-coupled receptor kinases. ACS Chem Biol. 10:246-256.

Lyon, A. M., Begley, J. A., Manett, T. D., Tesmer, J. J. G. 2014. Molecular mechanisms of phospholipase C β3 autoinhibition. Structure 22:1844-1854.

Homan, K. T., Wu, E., Cannavo, A., Koch, W. J., Tesmer, J. J. G. 2014. Identification and characterization of amlexanox as a G protein-coupled receptor kinase 5 inhibitor. Molecules 19:16937-16949.

Beautrait, A., Michalski K. R., Lopez, T. S., Mannix, K. M., McDonald, D. J., Cutter, A. R., Medina, C. B., Hebert, A. M., Francis, C. J., Bouvier, M., Tesmer, J. J. G., Sterne-Marr, R. 2014. Mapping the putative G protein-coupled receptor (GPCR) docking site on GPCR kinase 2: insights from intact cell phosphorylation and recruitment assays. J  Biol Chem. 289:25262-25275.

Swanson, C. J., Ritt, M., Wang, W., Lang, M. J., Narayan, A., Tesmer, J. J. G., Westfall, M., Sivaramakrishnan, S. 2014. Conserved modular domains team up to latch-open active protein kinase Cα. J Biol Chem. 289:17812-17829.

Homan, K. T., Tesmer, J. J. G. 2014. Structural insights into G protein-coupled receptor kinase function. Curr Opin Cell Biol. 27:25-31.

Homan K. T., Wu, E., Wilson, M. W., Singh, P., Larsen, S. D., Tesmer, J. J. G. 2014. Structural and functional analysis of G protein-coupled receptor kinase inhibition by paroxetine and a rationally designed analog. Mol Pharm.  85:237-248.

Ding, B., Glukhova, A., Sobczyk-Kojiro, K., Mosberg, H. I., Tesmer, J. J. G., Chen, Z. 2014. Unveiling the membrane-binding properties of N-terminal and C-terminal regions of G protein-coupled receptor kinase 5 by combined optical spectroscopies. Langmuir 30:823-831.

Lyon, A. M., Taylor, V. G., Tesmer, J. J. G. 2014. Strike a pose: Gαq complexes at the membrane. Trends Pharmacol Sci. 35:23-30.

Yang, P., Glukhova, A., Tesmer, J. J. G., Chen, Z. 2013. Membrane orientation and binding determinants of G protein-coupled receptor kinase 5 as assessed by combined vibrational spectroscopic studies. PloS One 8:e82072. PMCID: 3838385

Vishnivetskiy, S. A., Ostermaier, M. K., Singhal, A., Panneels, V., Homan, K. T., Glukhova, A., Sligar, S. G., Tesmer, J. J. G., Schertler, G. F. X., Standfuss, J., Gurevich. 2013. Constitutively active rhodopsin mutants causing night blindness are effectively phosphorylated by GRKs but differ in arrestin-1 binding. Cell Signal. 25:2155-2162.

Lyon, A. M., Tesmer, J. J. G. 2013. Structural insights into phospholipase C-β function. Mol Pharmacol. 84:488-500.

Lyon, A. M., Dutta, S., Skiniotis, G., Tesmer, J. J. G. 2013. Characterization of phospholipase C-β3 complexes by x-ray crystallography and single particle electron cryo-microscopy. Trans Am Crystallogr Assoc.  http://www.amercrystalassn.org/2013-transactions_toc

Singhal, A., Ostermaier, M. K., Vishnivetskiy, S. A., Panneels, V., Homan, K. T., Tesmer, J. J. G., Veprintsev, D., Deupi, X., Gurevich, V. V., Schertler, G. F. X., Standfuss, J. 2013. Insights into congenital stationary night blindness based on the structure of G90D rhodopsin. EMBO Rep. 14:520-526.

Yang, P., Boughton, A. P., Homan, K. T., Tesmer, J. J. G., Chen, Z. 2013. Membrane orientation of Gαiβ1γ2 and Gβ1γ2 determined via combined vibrational spectroscopic studies. J Am Chem Soc. 135:5044-5051.

Nance, M. R., Kreutz, B., Tesmer, V. M., Sterne-Marr, R., Kozasa, T., Tesmer, J. J. G. 2013. Structural and functional analysis of the regulator of G protein signaling 2-Gαq complex. Structure 21:438-448.

Lyon, A. M., Dutta, S., Boguth, C. A., Skiniotis, G., Tesmer, J. J. G. 2013. Full-length Gαq-phospholipase C-β3 structure reveals reveals interfaces of the C-terminal coiled-coil domain. Nat Struct Mol Biol. 20:355-362.

Sterne-Marr, R., Baillargeon, A. I., Michalski, K. R., Tesmer, J. J. G. 2013. Expression, purification, and analysis of G-protein-coupled receptor kinases. Methods Enzymol. 521:347-366.

Homan, K. T., Glukhova, A., Tesmer, J. J. G. 2013. Regulation of G protein-coupled receptor kinases by phospholipids. Curr Med Chem. 20:39-46.

Thal, D. M., Homan, K. T., Chen, J., Wu, E. K., Hinkle, P. M., Huang, Z. M., Chuprun, J. K., Song, J., Gao, E., Cheung, J. Y., Sklar, L. A., Koch, W. J., Tesmer, J. J. G. 2012. Paroxetine is a direct inhibitor of G protein-coupled receptor kinase 2 and increases myocardial contractility. ACS Chem Biol. 7:1830-1839.

Tesmer, V. M., Lennarz, S., Mayer, G., Tesmer, J. J. G. 2012. Molecular mechanism for inhibition of G protein-coupled receptor kinase 2 by a selective RNA aptamer. Structure 20:1300-1309.

Tesmer, J. J. G., Nance, M. R., Singh, P., Lee, H. 2012. Structure of a monomeric variant of rhodopsin kinase at 2.5 Å resolution. Acta Crystallogr Sect F Struct Biol Cryst Commun. 68:622-625.

Edwards, B. S., Zhu, J., Chen, J., Carter, M. B., Thal, D. M., Tesmer, J. J. G., Graves, S. W., Sklar, L. 2012. Cluster cytometry for high-capacity bioanalysis. Cytometry A 81:419-429.

Orban, T., Huang, C.-C., Homan, K. T., Jastrzebska, B., Tesmer, J. J. G., Palczewski, P. 2012. Substrate-induced changes in dynamics of rhodopsin kinase (G protein-coupled receptor kinase 1). Biochemistry 51:3404-3411.

Tesmer, J. J. G. 2012. A GAIN in understanding autoproteolytic G protein-coupled receptors and polycystic kidney disease proteins. EMBO J. 31:1334-1335.

Gurevich, E. V., Tesmer, J. J. G., Mushegian, A., Gurevich, V. V. 2012. G protein-coupled receptor kinases: more than just kinases and not only for GPCRs. Pharmacol Ther. 133:40-69.

Narasimhan, D., Collins, G. T., Nance, M. R., Nichols, J., Edwald, E., Chan, J., Ko, M.-C., Woods, J. H., Tesmer, J. J. G., Sunahara, R. K. 2011. Subunit stabilization and polyethylene glycolation of cocaine esterase improves in vivo residence time. Mol Pharm. 80:1056-1065.

Aittaleb, M., Nishimura, A., Linder, M., Tesmer, J. J. G. 2011. Plasma membrane association of p63 Rho guanine nucleotide exchange factor (p63RhoGEF) is mediated by palmitoylation and is required for basal activity in cells. J Biol Chem. 286:34448-34456.

Lyon, A. M., Tesmer, V. M., Dhamsania, V. D., Thal, D. M., Gutierrez, J., Chowdhury, S., Suddala, K. C., Northup, J. K., Tesmer, J. J. G. 2011. An autoinhibitory helix in the C-terminal region of phospholipase C-β mediates Gαq activation. Nat Struct Mol Biol. 18:999-1005.

Huang, C.-C., Orban, T., Jastrzebska, B., Palczewski, K., Tesmer, J. J. G. 2011. Activation of G protein-coupled receptor kinase 1 involves interactions between its N-terminal region and its kinase domain. Biochemistry 50:1940-1949.

Huang, C.-C., Tesmer, J. J. G. 2011. Recognition in the face of diversity: interactions of the heterotrimeric G proteins and G protein-coupled receptor (GPCR) kinases with activated GPCRs. J Biol Chem. 286:7715-7721.

Thal, D. M., Yeow, R. Y., Schoenau, C., Huber, J., Tesmer, J. J. G. 2001. Molecular mechanism of selectivity among G protein-coupled receptor kinase 2 inhibitors. Mol Pharm. 80:294-303.

Boughton, A. P., Yang, P., Tesmer, V. M., Ding, B., Tesmer, J. J. G., Chen, Z. 2011. Heterotrimeric G protein β1γ2 subunits change orientation upon complex formation with G protein-coupled receptor kinase 2 (GRK2) on a model membrane. Proc Natl Acad Sci U S A. 108:E667-E673.

Bayburt, T. H., Vishnivetskiy, S. A., McLean, M. A., Morizumi, T., Huang, C.-C., Tesmer, J. J. G., Ernst, O. P., Sligar, S. G., Gurevich, V. V. 2011. Monomeric rhodopsin is sufficient for normal rhodopsin kinase (GRK1) phosphorylation and arrestin-1 binding. J Biol Chem. 286:1420-1428.

Boguth, C. A., Singh, P., Huang, C.-C, Tesmer, J. J. G. 2010. Molecular basis for activation of G protein-coupled receptor kinases. EMBO J. 29:3249-3259.

Shankaranarayanan, A., Boguth, C. A., Lutz, S., Vettel, C., Uhlemann, F., Aittaleb, M., Wieland, T., Tesmer, J. J. G. 2010. Gαq allosterically activates and relieves autoinhibition of p63RhoGEF. Cell Signal. 22:1114-1123.

Tesmer, J. J. G. 2010. The quest to understand heterotrimeric G protein signaling. Nat Struct Mol Biol. 17:650-652.

Narasimhan, D., Nance, M. R., Gao, D., Ko, M.-C., MacDonald, J., Tamburi, P., Yoon, D., Landry, D. M., Woods, J. H., Zhan, C.-G., Tesmer, J. J. G., Sunahara, R. K. 2010. Structural analysis of thermostabilizing mutations of cocaine esterase. Protein Eng Des Sel. 23:537-547.

Brim, R. L., Nance, M. R., Youngstrom, D. W., Narasimhan, D., Zhan, C.-G., Tesmer, J. J. G., Sunahara, R. K., Woods, J. H. 2010. A thermally stable form of bacterial cocaine esterase: a potential therapeutic agent for treatment of cocaine abuse. Mol Pharm. 77:593-600.

Tesmer, J. J. G., Tesmer, V. M., Lodowski, D. T., Steinhagen, H., Huber, J. 2010. Structure of human G protein-coupled receptor kinase 2 in complex with the kinase inhibitor balanol. J Med Chem. 53:1867-1870.

Talbot, J. N., Roman, D. L., Clark, M. J., Roof, R. A., Tesmer, J. J. G., Neubig, R. R., Traynor, J. R. 2010. Differential modulation of µ-opioid receptor signaling to adenylyl cyclase by regulators of  G protein signaling proteins 4 or 8 and 7 in permeabilised C6 cells is Gα subtype dependent. J Neurochem. 112:1026-1034.

Aittaleb, M., Boguth, C. A., Tesmer, J. J. G. 2010. Structure and function of heterotrimeric G protein-regulated Rho guanine nucleotide exchange factors. Mol Pharmacol. 77:111-125.

Aittaleb, M., Gao, G., Evelyn, C. R., Neubig, R. R., Tesmer, J. J. G. 2009. A conserved hydrophobic surface of the LARG pleckstrin homology domain is critical for RhoA activation in cells. Cell Signal. 21:1569-1578.

Huang, C.-C., Yoshino-Koh, K., Tesmer, J. J. G. 2009. A surface of the kinase domain critical for the allosteric activation of G protein-coupled receptor kinases. J Biol Chem. 284:17206-17215.

Sterne-Marr, R., Leahey, P. A, Bresee, J. E., Dickson, H. M., Ho, W., Ragusa, M. J., Donnelly, R. M., Amie, S. M., Krywy, J. A., Brookins-Danz, E. D., Orakwue, S. C., Carr, M. J., Yoshino-Koh, K., Li, Q., Tesmer, J. J. G. 2009. GRK2 activation by receptors: role of the kinase large lobe and carboxyl-terminal tail. Biochemistry 48:4285-4293. PMCID: 2744320

Shankaranarayanan, A., Thal, D. M., Tesmer, V. M., Roman, D. L., Neubig, R. R., Kozasa, T., Tesmer, J. J. G. 2008. Assembly of high order Gαq-effector complexes with RGS proteins. J Biol Chem. 283:34923-34934.

Tesmer, J. J. G. 2008. Guanylyl cyclase sees the light. J Biol. 7:31.

Singh, P., Wang, B., Madea, T., Palczewski, K., Tesmer, J. J. G. 2008. Structures of rhodopsin kinase in different ligand states reveal key elements involved in G protein-coupled receptor kinase activation. J Biol Chem. 283:14053-14062.

Lutz, S., Shankaranarayanan, A., Coco, C., Ridilla, M., Nance, M. R., Vettel, C., Baltus, D., Evelyn, C. R., Neubig, R. R., Wieland, T., Tesmer, J. J. G. 2007. Structure of Gαq-p63RhoGEF-RhoA complex reveals a pathway for the activation of RhoA by GPCRs. Science 318:1923-1927.

Gu, S., He, J., Ho, W.-T., Ramineni, S., Thal, D. M., Natesh, R., Tesmer, J. J. G., Hepler, J. R., Heximer, S. P. 2007. Unique hydrophobic extension of the RGS2 amphipathic helix domain imparts increased plasma membrane binding and function relative to other RGS R4/B subfamily members. J Biol Chem. 282:33064-33075.

Williams, S. L., Lutz, S., Charlie, N. K., Vettel, C., Ailion, M., Coco, C., Tesmer, J. J. G., Jorgensen, E. M., Wieland, T., Miller, K. G. 2007. Trio’s Rho-specific GEF domain is the missing Gαq effector in C. elegans. Genes Dev. 21:2731-2746.

Chen, X., Boughton, A. P., Tesmer, J. J. G., Chen, Z. 2007. In situ investigation of heterotrimeric G protein βγ subunit binding and orientation on membrane bilayers.  J Am Chem Soc. 129:12658-12659.

Li, Q., Nance, M. R., Kulikauskas, R., Nyberg, K., Fehon, R., Karplus, P. A., Bretscher, A., Tesmer, J. J. G. 2007. Self-masking in an intact ERM-merlin protein: an active role for the central α-helical domain. J Mol Biol. 365:1446-1459.

Lodowski, D. T., Tesmer, V. M., Benovic, J. L., Tesmer, J. J. G. 2006. The structure of G protein-coupled receptor kinase (GRK)-6 defines a second lineage of GRKs. J Biol Chem. 281:16785-16793.

Tesmer, J. J. G. 2006. Hitting the hot spots of cell signaling cascades. Science 312:377-378.

Kreutz, B., Yau, D. M., Nance, M. R., Tanabe, S., Tesmer, J. J. G., Kozasa, T. 2006. A new approach to producing functional Gα subunits yields the activated and deactivated structures of Gα12/13 proteins. Biochemistry 45:167-174. 

Tesmer, V. M., Kawano, T., Shankaranarayanan, A., Kozasa, T, Tesmer, J. J. G. 2005. Snapshot of activated G proteins at the membrane: the Gαq-GRK2-Gβγ complex. Science 310:1686-1690.

Lodowski, D. T., Barnhill, J. F., Pyskadlo, R. M., Ghirlando, R., Sterne-Marr, R., Tesmer, J. J. G. 2005. The role of Gβγ and domain interfaces in the activation of G protein-coupled receptor kinase 2. Biochemistry 44:6958-6970.

Tesmer, J. J. G. 2005. A seminal study of soluble adenylyl cyclase. Nat Struct Mol Biol. 12:7-8.

Kristelly, R, Gao, G., Tesmer, J. J. G. 2004. Structural determinants of RhoA binding and nucleotide exchange in leukemia-associated Rho guanine-nucleotide exchange factor. J Biol Chem. 279:47352-47362.

Day, P. W., Tesmer, J. J. G., Sterne-Marr, R., Freeman, L. C., Benovic, J. L., Wedegaertner, P. B. 2004. Characterization of the GRK2 binding site of Gαq. J Biol Chem. 279:53643-53652.

Sterne-Marr, R., Dhami, G. K., Tesmer, J. J. G., Ferguson, S. S. G. 2004. Characterization of GRK2 RH domain-dependent regulation of GPCR coupling to heterotrimeric G proteins. Methods Enzymol. 390:310-336.

Kristelly, R., Earnest, B. T., Krishnamoorthy, L., Tesmer, J. J. G. 2003. Preliminary structure analysis of the DH/PH domains of leukemia-associated RhoGEF. Acta Crystallog D Biol Crystallogr. 50:1859–1862.

Lodowski, D. T., Pitcher, J. A., Capel, W. D., Lefkowitz, R. J., Tesmer, J. J. G. 2003. Keeping G proteins at bay: a complex between G protein-coupled receptor kinase 2 and Gβγ. Science 300:1256-1262.

Lodowski, D. T., Barnhill, J. F., Pitcher, J. A., Capel, W. D., Lefkowitz, R. J., Tesmer, J. J. G. 2003. Purification, crystallization and preliminary X-ray diffraction studies of a complex between G protein-coupled receptor kinase 2 and Gβ1γ2. Acta Crystallogr D Biol Crystallogr. 59:936-939.

Sterne-Marr, R., Tesmer, J. J. G., Day, P. W., Stracquatanio, R. P., Cilente, J. E., O'Connor, K. E., Pronin, A. N., Benovic, J. L., Wedegaertner, P. B. 2003. G protein-coupled receptor kinase 2/Gαq/11 interaction. A novel surface on a regulator of G protein signaling homology domain for binding Gα subunits. J Biol Chem. 278:6050-6058.

Tesmer, J. J. G., Sunahara, R. K., Fancy, D. A., Gilman, A. G., Sprang, S. R. 2002. Crystallization of complex between soluble domains of adenylyl cyclase and activated Gsα. Methods Enzymol. 345:198-206.

Tesmer, J. J. G., Dessauer C. W., Sunahara, R. K., Murray, L. D., Johnson, R. A., Gilman, A. G., Sprang, S. R. 2000. Molecular basis for P-site inhibition of adenylyl cyclase.  Biochemistry 39:14464-14471.

Pitcher, J. A., Tesmer, J. J. G., Freeman, J. L. R., Capel, W. D., Stone, W. C., Lefkowitz, R. J. 1999. Feedback inhibition of G protein-coupled receptor kinase 2 (GRK2) activity by extracellular signal-regulated kinases. J Biol Chem. 274:34531-34534.

Tesmer, J. J. G., Sunahara, R. K., Johnson, R. A., Gosselin, G., Gilman, A. G, Sprang, S. R. 1999. Two-metal-ion catalysis in adenylyl cyclase. Science 285:756-760.

Posner, B. A., Mukhopadhyay, S., Tesmer, J. J. G., Gilman, A. G., Ross, E. M. 1999. Modulation of the affinity and selectivity of RGS protein interaction with Gα subunits by a conserved asparagine/serine residue. Biochemistry 38:7773-7779.

Dessauer, C. W., Tesmer, J. J. G., Sprang, S. R., Gilman, A. G. 1999. The interactions of adenylate cyclases with P-site inhibitors. Trends Pharmacol Sci. 20:205-210.

Tesmer, J. J. G.,  Sprang, S. R. 1998. The structure, catalytic mechanism and regulation of adenylyl cyclase. Curr Opin Struct Biol. 8:713-719.

Sunahara, R. K., Beuve, A., Tesmer, J. J. G., Sprang, S. R., Garbers, D. L., Gilman, A. G. 1998. Exchange of substrate and inhibitor specificities between adenylyl and guanylyl cyclases. J Biol Chem. 273:16332-16338.

Dessauer, C. W., Tesmer, J. J. G., Sprang, S. R., Gilman, A. G. 1998. Identification of a Giα binding site on type V adenylyl cyclase. J Biol Chem. 273:25831-25839.

Sunahara, R. K., Tesmer, J. J. G., Gilman, A. G., Sprang, S. R. 1997. Crystal structure of the adenylyl cyclase activator G. Science 278:1943-1947.

Tesmer, J. J. G., Sunahara, R. K., Gilman, A. G., Sprang, S. R. 1997. Crystal structure of the catalytic domains of adenylyl cyclase in a complex with Gsα·GTPγS. Science 278:1907-1916.

Tesmer, J. J. G., Berman, D. M., Gilman, A. G., Sprang, S. R. 1997. Structure of RGS4 bound to AIF4-activated Giα1: stabilization of the transition state for GTP hydrolysis. Cell 89:251-261.

Tesmer, J. J. G., Klem, T. J., Deras, M. L., Davisson, V. J., Smith, J. L. 1996. The crystal structure of GMP synthetase reveals a novel catalytic triad and is a structural paradigm for two enzyme families. Nat Struct Biol. 3:74-86.

Tesmer, J. J. G., Stemmler, T. L., Penner-Hahn, J. E., Davisson, V. J., Smith, J. L. 1994. Preliminary X-ray analysis of Escherichia coli GMP synthetase: determination of anomalous scattering factors for a cysteinyl mercury derivative. Proteins 18:394-403.

Vermersch, P. S., Tesmer, J. J. G., Quiocho, F. A. 1992. Protein-ligand energetics assessed using deoxy and fluorodeoxy sugars in equilibrium binding and high resolution crystallographic studies. J Mol Biol. 226:923-929.

Vermersch, P. S., Lemon, D. D., Tesmer, J. J. G., Quiocho F. A. 1991. Sugar-binding and crystallographic studies of an arabinose-binding protein mutant (Met108Leu) that exhibits enhanced affinity and altered specificity. Biochemistry 30:6861-6866.

Vermersch P. S., Tesmer J. J. G, Lemon D. D., Quiocho F. A. 1990. A Pro to Gly mutation in the hinge of the arabinose-binding protein enhances binding and alters specificity. Sugar-binding and crystallographic studies. J Biol Chem. 265:16592-16603.

Book Section

Tesmer, J. J. G. 2016. Crystallographic pursuit of a protein-RNA aptamer complex. In: Nucleic Acid Aptamers, G. Mayer, Ed., Methods in Molecular Biology, Springer New York, 1380:151-160.

Tesmer, J. J. G. 2011. Activation of G protein-coupled receptor (GPCR) kinases by GPCRs. In: G Protein-Coupled Receptors: From Structure to Function, J. Giraldo, J.-P. Pin, eds., Royal Society of Chemistry, pp. 297-315 (RSC Drug Discovery Series No. 8).

Tesmer, J. J. G. 2009. Structure and function of regulator of G protein signaling homology domains. In: Progress in Molecular Biology and Translational Science, R. A. Fisher, ed., Academic Press, 86:75-113.

 



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