Picture of SHERMAN

LOUIS A. SHERMAN

Professor
LILY G-235
Phone: 765-494-8106

Personal Site: Link

Cyanobacteria have become wonderful and versatile model organisms for the study of photosynthesis, nitrogen fixation and responses to environmental stresses. Our research can help answer questions involved with environmental concerns, alternative energy uses (i.e., solar energy), and health concerns such as microbial toxins and the design of new drugs. The genomic sequence of one favorite cyanobacterium was completed a few years ago, and we now have the task of trying to determine the function of each of its 3,264 genes, ultimately to understand how a cell functions. The lab has successfully constructed microarrays ("gene chips")--this is an important technique in biology and all of the genes in an organism can be spotted on a glass slide about the size of a microscope slide. These microarrays are great for studying the activity of genes; this technique, along with the ability to make mutations in virtually every gene, is a key way for determining gene function under different environmental conditions. The genome sequence of another of our favorite cyanobacteria has recently been completed. This unicellular organism shows robust metabolic and circadian rhythms and performs photosynthesis and N2-fixation at different times of the day and night. This organism is key to a large project aimed at understanding the regulation of such processes and the assembly of membrane complexes. The genomes of 6 more Cyanothece species are being sequenced at DOE and will aid us in identifying important genes and processes for alternate energy uses.

Education

B.S., University of Chicago, 1965

Ph.D., University of Chicago, 1970

Postdoc, Cornell University, 1972

Awards

  • Lady Davis Fellowship, Hebrew University, Jerusalem, Israel

Other Activities

Grant Review/Study Section

  • DOE
  • NSF
  • USDA
  • NIH

Faculty Presentations

  • Differential regulation of environmental Stress genes under natural solar radiation in the cyanobacterium Synechocystis sp. 6803.  ABSCICON 2006.  Washington, DC, March 21-23, 2006.
  • Genome Sequence Analysis of Cyanothece sp. ATCC 51142, a Unicellular Nitrogen-fixing Cyanobacterium.  1st Annual Finishing the Genome in the Future Meeting, Santa Fe, NM, May 4-5, 2006.
  • Role of sigB on global gene regulation during the onset of early stationary phase in the cyanobacterium Synechocystis sp. PCC 6803.  106th Annual ASM Meeting, Orlando, Florida, May 21-25, 2006.
  • Sigma factors and environmental regulation of global gene expression in Synechocystis sp. PCC 6803.  Invited Talk at the 12th International Symposium on Phototrophic Prokaryotes, Pau, France, August 27 - Sept. 1, 2006.
  • Genetic Transformation and Mutagenesis in the Unicellular Diazotrophic Cyanobacterium Cyanothece sp. ATCC 51142.  12th International Symposium on Phototrophic Prokaryotes, Pau, France, August 27 - Sept. 1, 2006.
  • Microarray Analysis of the Genome-Wide Response to Alkaline pH in the Cyanobacterium Synechocystis sp. PCC 6803.  12th International Symposium on Phototrophic Prokaryotes, Pau, France, August 27 - Sept. 1, 2006.
  • Genomics and systems biology analysis of Cyanothece sp. ATCC 51142, a unicellular, diazotrophic cyanobacterium with robust metabolic rhythms.  Plenary lecture, 15th International Nitrogen Fixation Congress, Cape Town, South Africa, Jan. 21-28, 2007.
  • Genomics and genetic transformation in the unicellular, diazotrophic cyanobacterium, Cyanothece sp. ATCC 51142.  15th International Nitrogen Fixation Congress, Cape Town, South Africa, Jan. 21-28, 2007.
  • Gentectic transformation and recombination in the unicellular, diazotrophic cyanobacterium Cyanothece sp. ATCC 51142.  9th Cyanobacterial Molecular Biology Workshop, Delavan, Wisconsin, June 7-10, 2007.
  • The role of sigma factors in controlling global gene expression in light/dark transitions in the cyanobacterium Synechocystis sp. PCC 6803.  9th Cyanobacterial Molecular Biology Workshop, Delavan, Wisconsin, June 7-10, 2007.
  • Metabolic regulation of the cyanobacterium Cyanothece sp. ATCC 51142 when grown in light/dark or in continuous light.  9th Cyanobacterial Molecular Biology Workshop, Delavan, Wisconsin, June 7-10, 2007.
  • Fermentation ability of the cyanobacterium Cyanothece.  9th Cyanobacterial Molecular Biology Workshop, Delavan, Wisconsin, June 7-10, 2007.
  • Complete genome sequence of Cyanothece sp. ATCC 51142.  DOE Joint Initiative Meeting "Finishing Genomes in the Future."  Santa Fe, NM, June 16-20, 2007.
  • Metabolic regulation of the cyanobacterium Cyanothece sp. ATCC 51142 when grown in light-dark or continuous light.  IV European Phycological Conference, Oviedo, Spain, July 22-26, 2007.
  • Regulatory and structural changes throughout a day in the life of a cyanobacterium - Cyanothece sp. ATCC 51142.  Avron-Minerva Symposium on Photosynthesis, Jerusalem, Israel, Nov. 25-28, 2007.
  • The Genus Cyanothece, unicellular diazotrophic cyanobacteria that produce O2, fix N2 and that have highly versatile metabolic systems.  General Society for Microbiology, UK, Annual Meeting, Symposium on Cyanobacteria: What are they and what do they do?  Edinburgh, Scotland, April 1-5, 2008.
  • Anaerobic induction of normally cryptic psbA genes in cyanobacteria.  European Cyanobacterial Workshop, Prague, Czech Republic, Sept. 2008.
  • Anaerobic induction of normally cryptic psbA genes in cyanobacteria.  Photosynthesis in the Global Perspective, Indore, India, Nov. 2008.
  • Development of Cyanothece as a new model organism for biological hydrogen production.  GtL Workshop, Washington, D.C., Feb. 2009.
  • Better living through Cyanothece--unicellular diazotrophic cyanobacteria with highly versatile metabolic systems.  International Society for Photosynthetic Prokaryotes, Montreal, Canada, Aug. 2009.
  • Ferritin family proteins at the cross roads between iron homeostasis and oxidative stress.  International Society for Photosynthetic Prokaryotes, Montreal, Canada, Aug. 2009.
  • Global transcriptional response to low oxygen conditions and the role the histidine kinase, Hik31, in the cyanobacterium Synechocystis sp. PCC 6803.  International Society for Photosynthetic Prokaryotes, Montreal, Canada, Aug. 2009.
  • Regulation of autotrophic and heterotrophic metabolism in Synechocystis sp. PCC 6803 by two-component system clusters on both the chromosome and a plasmid.  International Society for Photosynthetic Prokaryotes, Montreal, Canada, Aug. 2009.
  • CO2 fixation and carbon storage in cyanobacteria.  GCEP Workshop on Biological Capture and Utilization of CO2, St. Louis, MO, Sept. 2009.
  • Towards an understanding of proteins that govern the structure and function of Synechocystis sp. PCC 6803 and multiple Cyanothece strains.  DOE GtL Users Meeting, Washington, D.C., Feb. 7-9, 2010.
  • Development of Cyanothece as a new model organism for photobiological hydrogen production.  DOE GtL Users Meeting, Washington, D.C., Feb 7-9, 2010.
  • Genome sequences of the unicellular, N2-fixing cyanobacterial genus Cyanothece.  DOE Genome Meeting, Walnut Creek, CA, March 24-27, 2010.
  • Development of genetic and molecular tools for the improvement of hydrogen production in Cyanothece sp. Strain PCC 7822.  10th Molecular Biology of Cyanobacteria Workshop, Lake Arrowhead, CA, June 11-15, 2010.
  • Cyanobacteria in Space—the role of synthetic biology in the development of specialized cyanobacteria for long-term space flight.  NASA Synthetic Biology, NASA Ames Research Center, CA, Oct. 30, 2010. 
  • Development of Cyanothece as a New Model Organism for Photobiological Hydrogen Production. Genomic Sciences and Bioenergy Meeting, Washington, DC, April 11-14, 2011. 
  • Regulations of Autotrophic and Heterothrophic Metabolism in Synechocystis sp. Strain PCC 6803 By HIK31 Operons on both the Chromosome and Plasmid   PSYSX. American Society for Microbiology Meeting, New Orleans, LA, May 21-24, 2011.
  • Cyanothece, unicellular diazotophic cyanobacteria: physiology, genetics and modeling. Workshop on Cyanobacterial Metabolism: Models & Validation, Lake Geneva, WI, June 26-29, 2011. 
  • Carbon Storage and metabolic engineering of H2 production in the unicellular, diazotroph Cyanothece. 8th  European Workshop Molecular Biology of  Cyanobacteria, Naantali, Finland, August 28-September 1, 2011. 
  • Biofuels from the unicellular, diazotrophic cyanobacteria cyanothece. ESF-BU-CEBITEC Conference “Microorganoms for Bio-fuel Production from Sunlight” Bielefeld, Germany, September 18-21, 2011.

 

Conferences

  • 6th NCSE Conf: Energy for a Sustainable and Secure Future, January 2006
  • AAAS Meeting and Symposium on Grand Challenges, St. Louis, MO, February 2006
  • 1st Annual Finishing Genomes in the Future Meeting, Santa Fe, NM, May 2006
  • 106th ASM Meeting, Orlando, FL, May 2006
  • 12th International Symposium on Phototrophic Prokaryotes, Pau, France, August 2006
  • 15th International Nitrogen Fixation Congress, Cape Town, South Africa, January 2007
  • Membrane Biology Grand Challenge Workshop, St. Louis, MO, April 2007
  • 9th Cyanobacterial Workshop on Molecular Biology, Delavan, WI, June 2007
  • 2nd Annual Finishing Genomes in the Future Meeting, Santa Fe, NM, June 2007
  • Avron-Minerva Symposium on Photosynthesis, Jerusalem, Israel, November 2007
  • Society for General Microbiology, Edinburgh, Scotland, April 2008
  • Photosynthesis in the Global Perspective, Indore, India, November 2008
  • DOE Genomics Sciences Meeting, Washington, DC, February 2009
  • University of Stockholm, Department Plant Biology, May 2009
  • 13th International Symposium on Phototrophic Prokaryotes, Montreal, Canada, August 2009
  • Algal CO2 Capture, St. Louis, MO, September 2009
  • DOE Genomics Sciences Meeting, Washington, DC, February 2010
  • DOE Joint Genome Initiative Genomics Meeting, Walnut Creek, California, March 2010
  • Sequencing, Finishing and Analysis in the Future, Santa Fe, New Mexico, June 2010
  • 10th Molecular Biology of Cyanobacteria Workshop, Lake Arrowhead, California, June 2010
  • NASA - Synthetic Biology, Ames, California, October 2010
  • Modeling Cyanobacteria in silico: Virtual Bioreactor, Mikulov, Czech Republic, September 2010
  • DOE Genomics Sciences/Biofuels Meeting, Washington, DC, April 2011
  • ASM Meeting, New Orleans, Louisiana, May 2011
  • Workshop on Cyanobactrial Metabolism, Lake Geneva, Wisconsin, June 2011
  • 8th European Workshop on Mol Biol Cyanobacteria, Naantali, Finland, August 2011
  • ESF Conf. Microorganisms for BioFuel Production, Bielefeld, Germany, September 2011

Professional Faculty Research

(microbiology) Photosynthetic membranes - structure, function, and assembly in cyanobacteria; functional genomics and impact of environmental factors on photosynthesis; analysis of global regulation with microarrays; regulation of photosynthesis and N2-fixation in unicellular cyanobacteria.

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