Course Descriptions

BIOL 69500 Microsopy For Life Scientists

Session Offered:

Spring Credit 2

Prerequisites:

graduate students

Description:

Purpose of the course:

 

Many life scientists are using advanced imaging techniques without fully understanding the underlying principles of sample preparation, imaging formation, and data analysis. The goal of this course is to provide graduate students with a fundamental knowledge in various aspects of light microscopy, including modern fluorescence imaging techniques, digital image processing and analysis with emphasis on how to optimally image and analyze biological samples. Completing this course will help students to make better use of available techniques and design new experiments.

Major topics include:

1.  How light travels through a light microscope to form an image

2.  Image formation in bright-field, phase contrast, DIC, epifluorescence, and confocal microscopy

3.  How to use Image J (http://rsbweb.nih.gov/ij/) for automated image processing and analysis

 

Additional topics include how to handle and adjust common hardware components, how to recognize and correct common image artifacts, and how to prepare biological samples for best imaging.  Concept-based lectures are complemented with hands-on laboratories.  Coverage is from a non-mathematical perspective, and no knowledge of math of physics is expected.

Instructor(s):
DANIEL SUTER

E-mail:
dsuter@purdue.edu

Textbook(s):

no text is required for this course

Course Format:

CRN

Sec

Type

Days

Time

Location

14523

022

Lec

WF

11:30-12:20

LILY G458

 



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