BIOL 55101 Theory Of Molecular Methods
Session Offered:Spring Credit 3.0
This course will introduce upper-level undergraduate students and graduate students to the theory and practice of many commonly used molecular biology methods. Emphasis will be placed on understanding the theoretical basis for these techniques, their utility in various circumstances, and their limitations. Although taught as a lecture, students should be able to take this knowledge back to the laboratory to help them in their research efforts. The course will be as close as possible to a laboratory experience without being in an actual laboratory setting. This course is designed to prepare students for other in-depth scientific courses, for graduate rotations, and for future laboratory work. Exams will be experimental in nature (e.g., design an experiment to do something or in this type of situation, which technique/vector/etc. would best be used, and why). Permission of instructor required.
Topics to be covered will include:
a. Renaturation kinetics (including how to set stringency to look at gene
families, setting PCR primers, etc.)
b. Blotting strategies (Southern, Northern, Western, South-western, far
c. Recombinant DNA (how to choose the right type of vector to get the job
done, differences between selection and screening, different types of
marker systems, non-restriction enzyme cloning, etc.)
d. Nuclear run-on transcription (to look at rates of RNA synthesis) vs.
Northerns and RT-PCR.
e. Protein-DNA interaction systems (gel mobility shift, DNAse protection,
f. Protein-protein interaction systems (in vivo and in vitro)
g. Regulated/inducible gene expression systems
h. Genome engineering (meganucleases, zinc finger enzymes, TALEs, CRISPR
i. Recombination marker systems
j. Mutagenesis systems
k. Gene replacement strategies
l. Next generation genome sequencing
n. Degradome sequencing
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