Markey Center for Structural Biology





Picture of WANNER

BARRY L. WANNER

Professor
LILY 1-225
Phone: 765-494-8034

All cells have sensory proteins decorating their cell surfaces. These bioantennae detect environmental signals and transmit this critical information to cytosolic effector proteins, which trigger appropriate cellular responses. Most bacteria and some plant and animal cells use two-component regulatory systems for many of these signaling processes. These biosensors are comprised of a "sensory kinase" protein and a partner "response regulator" protein. This is an extremely effective means of communication; large families of homologous sensory kinase proteins and response regulatory proteins have been identified. We are studying the biological, biochemical, and molecular properties of several two-component systems, including the biosensors for inorganic phosphate, the antibiotic vancomycin, and some that are key for normal and pathogenic interactions between bacteria and human cells. We are also examining the cross regulation between different signaling systems that is important in global control of cell growth and metabolism. In addition, we are now doing highly integrative systems biology research on the E. coli model cell with the long-term goal of understanding how all of the metabolic, regulatory, and structural features come together to form a living cell. 

Education

Ph.D., Michigan, 1975

Other Activities

  • co-founder and co-Editor-in-Chief, BMC Microbial Informatics and Experimentation 2010-on
  • Hiroshima University, Hiroshima, Japan, October 13, 2003. Presented informal talks on signal transduction and polyphosphate metabolism.
  • International Workshop for Escherichia coli Towards New Biology in the 21st Century, Awaji-shima, Japan, October 15-17, 2003. Poster presentation: "Computational prediction and experimental validation of iron and phosphate gene regulatory sites in the Escherichia coli K-12 and Pseudomonas aeruginosa PA01 genomes," coauthored by S.-I. Jung, K.S. Setty, K. Siddiqi, L. Zhou, B.L. Wanner, and D. Kihara.
  • Nara Institute, Nara, Japan on October 18-22, 2003. Met with colleagues.
  • Bioinformatics Center, Kyoto University, Kyoto on October 21 and 22, 2003. Met with colleagues
  • "Facilitating Microbial Research Through Advanced Data Management Infrastructure," NSF sponsored workshop, Arlington, Virginia, October 27 to 29, 2003. Served as a panel member.
  • First International Workshop on Systems Biology of Yeast, Washington University, St. Louis, Missouri, November 9, 2003
  • E. coli Annotation Workshop, Marine Biological Laboratory, Woods Hole, Massachusetts, November 14-18, 2003.
  • UK Biotechnology and Biological Sciences Research Council (BBSRC) Grantholders Scoping Workshop on Biological Impacts of Genomic and Post-genomic Information in Microbes (BIG AIM), Lincolnshire, England, March 24-25, 2004. Served as outside consultant.
  • Advisory Board Meeting of the E. coli Encyclopedia EcoCyc Database, Stanford Research Institute, Menlo Park, California, May 8, 2004.
  • Beckman Center, Stanford University of Medicine, Palo Alto, California, May 7-13, 2004. Conducted collaborative research.
  • Met with colleagues at Korea Advanced Institute of Science and Technology (KAIST), Daegon, Korea on June 25, 2004, and with colleagues at Konkuk University and Yonsei University, Seoul, Korea on June 26, 2004.
  • Met with colleagues from the National Defense Academy, Tokyo, Japan, on June 27 and 28 and with colleagues at Nara Institute, Nara, Japan, on June 29, 2004 Institute of Molecular Biosciences, University of Queensland, Brisbane, Australia, July 4, 2004.
  • International E. coli Alliance planning meeting, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland, October 7 and 8, 2004.
  • 1st FEBS Advanced Lecture Course Planning Meeting on "Systems Biology: From Molecules & Modeling to Cells," which will be held in Gosau, Austria, in March 2005 (http://www.FEBSsysbio.net/). He also met with research collaborators at the Max Planck Institute for Developmental Biology, Tuebingen, Germany, October 14-15, 2004.
  • NIH Roadmap, Metabolomics Technology Development Annual Investigators Meeting (organized by NIH), Bethesda, Maryland, January 25, 2005.

 Grant Review/Study Section

  • NSF (member: 6 meetings)
  • NIH MBC1 (member: 10 meetings)
  • NIH MBC1 (chair: 9 meetings)
  • NIH PCMB (member: 1 meeting)
  • NIH Special Emphasis Panels (member: 4 meetings)

Faculty Presentations

  • "Application of molecular genomics tools in studies of two-component signaling in E. coli" and "Concluding Remarks and Future Aspects," (meeting summary), International Workshop for Escherichia coli Towards New Biology in the 21st Century, Awaji-shima, Japan, October 15 -17, 2003.
  • Stochastic changes in expression for a gene controlled by the PhoB/PhoR two-component signal transduction system," Keynote Seminar , UK Biotechnology and Biological Sciences Research Council (BBSRC) Grantholders Scoping Workshop on Biological Impacts of Genomic and Post-genomic Information in Microbes (BIG AIM), Lincolnshire, England, March 24 , 2004.
  • "Stochastic changes in expression for a gene controlled by the PhoB/PhoR two-component signal transduction system." Cardiff University, Cardiff, Wales, March 26, 2004.
  • "Stochastic changes in expression for a gene controlled by the PhoR/PhoB two-component regulatory system," ASM Conference on Integrating Metabolism and Genomics (IMAGE), Montreal, Quebec, Canada, April 30 - May 3, 2004.
  • "Genetic and growth control of ppk-ppx operon expression in E. coli K-12," Department of Biochemistry, Stanford University School of Medicine, Palo Alto, California, May 9, 2004.
  • "Molecular genomics tools for E. coli systems biology research," 2nd Conference of the International E. coli Alliance (IECA), Banff, Alberta, Canada, June 18, 2004.
  • Keynote Address "Two-component signal transduction by phosphate and vancomycin in bacteria," Korean Society of Microbiology and Biotechnology Symposium, Seoul, Korea, June 23, 2004.
  • "Stochastic changes in expression for a gene controlled by the PhoR/PhoB two-component regulatory system," Cell Signaling Symposium, Pai Chai University, Daegon, Korea, June 23, 2004.
  • "Stochastic changes in expression for a gene controlled by the PhoR/PhoB two-component regulatory system," Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daegon, Korea, June 25, 2004.
  • "Stochastic changes in expression for a gene controlled by the PhoR/PhoB two-component regulatory system," Konkuk University, Seoul, June 25, 2004.
  • "Stochastic changes in expression for a gene controlled by the PhoR/PhoB two-component regulatory system," Nagoya University, Osaka University, and Nara Institute, Japan on June 27 to July 2, 2004.
  • "Stochastic changes in expression for a gene controlled by the PhoR/PhoB two-component regulatory system," the National Institute for Basic Biology (NIBB), Okazaki, Japan, June 30, 2004.
  • "Stochastic changes in expression for a gene controlled by the PhoR/PhoB two-component regulatory system," Symposium on Integrative Bioinformatics - Aspects of the Virtual Cell, Dagstuhl, Germany, July 4-9, 2004.
  • "Stochastic changes in expression for a gene controlled by the PhoR/PhoB two-component regulatory system," GlaxoSmithKline, Upper Merion, Pennsylvania, August 10, 2004.
  • "Stochastic changes in expression for a gene controlled by the PhoR/PhoB two-component regulatory system," Bioengineering Program, University of Minnesota, Minneapolis, Minnesota, October 28, 2004.
  • "Stochastic changes in expression for a gene controlled by the PhoR/PhoB two-component regulatory system," Department of Biological Sciences, University of Maryland, Baltimore, October 21, 2004.

Conferences

  • E. coli Functional Genomics Conference, Osaka, Japan, October 8-21, 2003.
  • 4th International Conference on Systems Biology, November 5-9, 2003.
  • Pacific Symposium on Biocomputing 2004, North Kona, Big Island, Hawaii, January 5-9, 2004.
  • Genomes 2004: International Conference on the Analysis of Microbial and other United Kingdom, April 14-17, 2004. Poster presentation: "Two-step site- Genomes, Wellcome Trust Conference Center, Hinxton, Cambridgeshire, directed mutagenesis of chromosomal genes in Escherichia coli using PCR products" by Kirill A. Datsenko and Barry L. Wanner.
  • ASM Conference on Integrating Metabolism and Genomics (IMAGE), Montreal, Quebec, Canada April 30-May 3, 2004. Chaired the session on Complex Regulatory Systems.
  • First Annual Indiana Bioinformatics Conference, Indiana University School of Medicine Center for Computational Biology and Bioinformatics, Indianapolis, Indiana, May 27, 2004.
  • 2nd Conference of the International E. coli Alliance (IECA), Banff, Alberta, Canada, June 18-22, 2004. Serve as co-chair.
  • ISMB 2004 Conference, Glasgow, Scotland, July 31-August 5, 2004. Systems Biology of E. coli .Session organizer.
  • 12th International Conference on Intelligent Systems for Molecular Biology and the 3rd European Conference on Computational Biology, Glasgow, United Kingdom, July 30 - August 5, 2004.
  • 5th Annual International Conference on Systems Biology (ICSB 2004), Heidelberg, Germany, October 9 to 13, 2004. Workshop participant: "Modeling of Metabolic and Genetics Networks" and "Live Standards in Systems Biology and Protein Functional Parameters for Systems Biology Approaches and Simulation," in the Curation Meeting for the Systems Biology Markup Language Working Group, and presented a poster entitled: "Systematic Approaches for the Modeling of E. coli" by H. Mori and N. Yamamoto (Nara Institute, Japan), T. Baba (Keio Univ., Japan), and K. A. Datsenko, L. Zhou, and B. L. Wanner (Purdue Univ.).
  • 11th Annual Midwest Pathogenesis Conference, Michigan State University, East Lansing, Michigan, October 1 to 3, 2004. Poster presentation by Amanda Jiang and Jie Shao : "Systematic Approach to Identify Transcriptional Regulators of Different Genes" (co-authored by Amanda Y. S. Jiang, Jie Shao, Tomoya Baba, Hirotada Mori, and Barry L. Wanner, Purdue University, USA and Keio University, Japan)
  • Midwest Prokaryotic Cell and Molecular Biology Meeting, Loyola University, Chicago, Illinois, November 6, 2004.

Professional Faculty Research

(Molecular genetics) Molecular genetics; transmembrane signal transduction; cellular microbiology.