We have recently begun to work on a project that requires us to find out whether a zebrafish larva can see or not. To this end, we have built a small machine to check whether the larvae show optokinetic response (OKR), a visual behaviour.
This video shows the OKR machine, which is essentially a drum with black and white stripes that the rotating direction is controlled by a motor.
The fish larvae will be immobilized by thick solution in a Petri dish, which will be put inside the drum. The larvae with normal vision will be able to track the rotation and move their eye balls. In this video, the bottom larva shows a normal OKR response, while the top one, a blind larva caused by a genetic mutation, does not show the OKR response. An an bubble is put on the left to reflect the direction of the stripe movement.
During the process of fabricating this machine, we had come across with another cheaper way to do the same thing. There is a type of ancient Chinese lantern that part of outside drum will move due to heat convection generated by the light (A picture can be found in this Chinese article). We bought a contemporary version that the moving drums are driven by motor. The type that we bought has the moving mechanism for rotating in opposite direction. We then took apart the lantern and used the moving mechanism to drive the opposite rotating stripes. See the following video for the moving mechanism of this alternative version of OKR.
Even though it is not perfect, it works! The most amazing part is the difference in the cost of fabrication.
- OKR made from Chinese Lantern: ~ $US 3 (for buying the Chinese Lantern)
- OKR that is properly made as shown in the first video: ~$US 150 (for materials) + ~$600 (for labor) = ~ $800 (and that does not include the controller box) !!!
That is actually another example of the difference in the cost structure in doing research between the East and the West!
I also had a lot of fun sourcing other cheap parts for the final setup. For example, I have bought a very decent eye-piece camera for less than $40 (the price seems to have gone up a bit since then… but is still very cheap) to capture the video of larval eye movement as shown in the second video. I have also bought a very economical ring light from AmScope for less than $60 for illuminating the drum area finally.
We are going to use this assay to identify fish with eye problems and then characterize the underlying molecular defects. That will help us study and find cures for the same diseases in human.
