The Lilly Hall Zeiss LSM 710 microscope is a state-of-the-art laser scanning confocal microscope well-suited to imaging live cells as well as standard fixed immunofluorescence samples. It is equipped with laser lines at 405, 458, 488, 514, 561 and 633 nm and a 34 channel spectral detector that enables simultaneous imaging of multiple fluorescent proteins. The inverted platform is adapted for a variety of cell culture chambers, and includes a full incubator with CO2 and temperature control. It is outfitted with 40X and 63X apochromat water immersion lenses that are corrected for live cell imaging in culture medium, as well as a 63X planapochromat lens appropriate to fixed material in standard mounting medium. Zeiss Zen software enables advanced imaging including FRET, FRAP, fluorescent protein photoactivation, time lapse and 3-dimensional imaging. The microscope computer is on the university network and transferring image files to your lab computers is simple.
If you think the scope could be useful for your work, please contact Dr. Don Ready (dready@purdue.edu) and we’ll get you started.