BIOL 44201 Introductory Module: Protein Expression

Session Offered:
Fall Credit 2.0

Prerequisites:

Pre-requisite:  BIOL 23200 or 27100 and 24200 or 28100.

Description:

This is a project-oriented course designed to give the student exposure to laboratory research through a series of five-week modules. During this five-week introductory module, the students will learn the basics of expression, isolation, and characterization of recombinant proteins in E. coli using SDS-PAGE and Western blotting. In the first half of the course, students work initially with one recombinant DNA construct and then are given a second construct to use to identify the protein expressed. *Note: additional hours as needed for particular experiments.

Instructor(s):

Susan Karcher


E-mail:
karcher@purdue.edu

Textbook(s):

~No textbook required / Lab Manual is available on BlackBoard~

Course Format:

CRN

Sec

Type

Days

Time

Location

 50440

001

LAB

MW

1:30-5:20

LILY 2132

6332

006

LAB

TTH

1:30-5:20

LILY 2132

  50441

002

LABPREP

Monday

8:30-9:20

LILY G401



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