Department of Biological Sciences

Session Offered:

Prerequisites:

BIOL 23200 or 27100 and 24200 or 28100. Course Restriction:  Must be enrolled in the College of Science

Description:

In this five-week module, students will learn how to prepare specimens for viewing by fluorescence microscopy.  Specimens include mammalian tissue culture cells and zebrafish embryos.  The emphasis will be on the staining of the cytoskeleton in these preparations, utilizing antibodies and fluorescent probes.  The specimens will be viewed by wide-field and confocal laser scanning fluorescence microscopy.  Images will be acquired, processed and analyzed by modern computational methods.  Students will learn the basics of cell culture, immunocytochemistry, fluorescence microscopy, digital image processing and data analysis. At the end of the course, students prepare a PowerPoint presentation of their images and data.

Instructor(s):

Daniel M. Suter

E-mail:

Textbook(s):

~There is no textbook required for this course~

Grading:

Grading will be based on your performance, participation for the lab including making 4 flow charts, your lab notebook, a quiz, a lab report in PowerPoint format and its presentation at the end of the course.  You should keep a lab notebook during the course for taking notes and documentation of the experiments carried out

Grade Points:

Preparation/Flow charts   20
Attendance/Performance 20
Notebook                       20
PowerPoint Lab report     20

PowerPoint presentation  10
Quiz                              10
Total                           100

90-100 A
80-89 B
70-79 C
60-69 D

Course Format: