Department of Biological Sciences

Session Offered:

Prerequisites:

Concurrent prerequisite: BIOL 23100 or BIOL 27000 or BIOL 23000

Description:

Students are introduced to the molecular biology of the eukaryotic cell. In the first section students study topics in protein biology and biochemistry such as protein structure, function, isolation, molecular evolution, and the detection and molecular basis of human disease. Techniques used for these experiments include electrophoresis, chromatography, and the Western blot procedure. In the second section students localize enzymes in plant and animal cells, perform cell fractionation procedures, and study the properties of specific cell-surface receptor. Experiments on the properties and structure of DNA are presented in the final section of the course. These exercises stress the organization and complexity of the genome, gene function and regulation, and the structure of the eukaryotic chromosome. Techniques include restriction nuclease mapping and basic DNA cloning techniques. Students also carry out an independent research project of their own design.

Instructor(s):

John Anderson

E-mail:

Textbook(s):

Required
Text 1: A Laboratory Course in Cell and Molecular Biology
Year/Edition: Current Diagrams and Class Notes
ISBN Number:
Author(s): J.N. Anderson
Publisher:

May be purchased at Boiler Copy Center

Notes:

Exam information is available on-line via:
https://roomschedule.mypurdue.purdue.edu/Timetabling/exams.do

Course Format: