Graduate Studies
BIOL 54200 DNA Sequencing Lab
Offering:
Fall, Credit 1.0Prerequisites:
BIOL 23200 or 27100 and BIOL 24200 or 28100. Note course restriction: Must be enrolled in the College of Science.
Description:
In this lab module pairs of students prepare, run, and detect DNA sequencing ladders on miniaturized fast-running sequencing gels. Sequencing reactions are prepared from single-stranded and double-stranded DNA templates. The theory of DNA sequencing is taught, and experience is gained in manually reading the sequence from the sequencing ladders consisting of A,T,G and C lanes. Students learn how to connect to the National Center for Biotechnology Information (NCBI) and do BLAST sequence comparisons of the sequences that they read with DNA sequences in the national database GenBank.
Instructor(s):
Arnold SteinE-mail:
steina@purdue.eduTextbook(s):
-No textbook required for this course/lab manual provided.-
Course Format:
BIOL 54200 - This course meets Wks. 6-10. (Sep 28-Nov 3):
|
CRN |
Instructional Type |
Day |
Time |
Room(s) |
|
12491 |
4-hr Laboratory |
TTH |
Sec 07-1:30-5:20 |
LILY 2132 |
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