Graduate Studies


BIOL 50000 Introductory Module: Protein Expression

Offering:
Fall, Credit 2.0

Prerequisites:

BIOL 23200 or 27100 and BIOL 24200 or 28100.  Note course restriction:  Must be enrolled in the College of Science.

Description:

Biology 50000 modules are project-oriented courses designed to give the student exposure to laboratory research through a series of five-week modules. During this five-week introductory module, the students will learn the basics of expression, characterization and purification of recombinant proteins in E. coli using SDS-PAGE, Western blot and affinity purification. In the first half of the course, students work initially with a known recombinant DNA construct to learn the essential lab skills. In the second half of the course, students will finish a final project with an unknown construct to identify and purify the protein expressed.

Instructor(s):
Zhiqing Wang 

E-mail:
wang83@purdue.edu

Textbook(s):

-Lab Manual written by instructors purchased at CopyMat-

Course Format:

BIOL 50000 is a linked course and meets Wks. 1-5 (Aug 24-Sep 27).  You must choose from:

CRN

Instructional Type

Day

Time

Room(s)

12482

50-min LabP

M

Sec 03-10:30-11:20

LILY G401

12480-12481

4-hr Laboratory

MW or TTH

Sec 01-1:30-5:20
or
Sec 2 1:30-5:20

LILY 2132



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