Graduate Studies


BIOL 50000 Introductory Module: Protein Expression

Offering:
Summer May 17-Jun 18, Credit 2.0

Prerequisites:

Pre-requisite:  BIOL 23200 or 27100 and 24200 or 28100. Course restriction:  Must be enrolled in the College of Science.

Description:

Biology 50000 modules are project-oriented courses designed to give the student exposure to laboratory research through a series of five-week modules. During this five-week introductory module, the students will learn the basics of expression, characterization and purification of recombinant proteins in E. coli using SDS-PAGE, Western blot and affinity purification. In the first half of the course, students work initially with a known recombinant DNA construct to learn the essential lab skills. In the second half of the course, students will finish a final project with an unknown construct to identify and purify the protein expressed.

Instructor(s):
Susan Karcher 

E-mail:
karcher@purdue.edu

Textbook(s):

Lab Manual purchased in class.

Course Format:

BIOL 50000 is a linked course.  You must choose from:

CRN

Instructional Type

Day/Time/Room

10422

Laboratory

MW   (LILY 2132)

10421

Laboratory Prep

M 9:50-10:50 (LILY 2130)



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